Pollen and pollen rain in criminal investigations

DEFINITIONS: Pollen is the male reproductive structure of all flowering plants that is transported by wind, water, or animals to the female flower. Pollen rain is the release of vast amounts of pollen by male flowering plants in an ecological landscape.

SIGNIFICANCE: Palynological analysis of pollen rain can be an important tool in crime investigations because it can help determine chronology and the type of ecological community within which an event may have occurred.

Pollen is the male gametophyte of a flowering plant. It originates in the anther of a flower, which consists of chambers called pollen sacs. Each pollen sac gives rise to microscopic grains of pollen. Pollen grains are not the equivalent of sperm in animals; rather, each pollen grain consists of at least two cells: a generative cell that produces the sperm to fertilize the female egg and a larger tube cell that provides the corridor to deliver the sperm to the egg.

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Transfer of pollen to the female flower is accomplished via wind, water, or animals; wind-borne pollen is known as pollen rain. To facilitate dispersal by wind, pollen is small, lightweight, unscented, and generally inconspicuous. The spores released by mosses, ferns, and fungi are also considered part of pollen rain, and the study of such spores is part of forensic botany.

The volume of pollen rain released annually in any given area is enormous, with pollen covering the landscape in a very fine layer. Despite the abundance of pollen, this coating is invisible, or nearly so, except with the aid of a microscope. Pollen may become lodged in respiratory tracts and can accumulate on clothes, hair, and the body. The pollen rain in a given area is called a pollen assemblage. Because each species of plant and fungus contributes its own unique pollen, such pollen assemblages are natural fingerprints of the vegetation in their areas; these fingerprints may differ significantly from one area to another.

Palynology is the examination and analysis of pollen assemblages. By comparing the pollen grains found on or in corpses, on suspects and their possessions, and on other objects, forensic palynologists can link particular persons with crime scenes based on the expected pollen rain for those areas.

Bibliography

Alotaibi, Sager S, et al. "Pollen Molecular Biology: Applications in the Forensic Palynology and Future Prospects: A Review." Saudi Journal of Biological Sciences , vol. 27, no. 5, May 2020, doi.org/10.1016/j.sjbs.2020.02.019. Accessed 19 Aug. 2024.

Coyle, Heather Miller, ed. Forensic Botany: Principles and Applications to Criminal Casework. Boca Raton, Fla.: CRC Press, 2005.

Ezegbogu, Mark O. "Identifying the Scene of a Crime Through Pollen Analysis." Science and Justice, vol. 61, no. 3, May 2021, doi.org/10.1016/j.scijus.2020.12.002. Accessed 19 Aug. 2024.

Faegri, Knut, Peter Emil Kaland, and Knut Krzywinski. Textbook of Pollen Analysis. 4th ed. New York: John Wiley & Sons, 1989.

Horrocks, Mark, and Kevan A. J. Walsh. “Forensic Palynology: Assessing the Value of the Evidence.” Review of Palaeobotany and Palynology 103 (1998): 69-74.