Forensic palynology

DEFINITION: Investigative technique using microscopic examination of the morphology of pollen grains associated with crime.

SIGNIFICANCE: Species-specific pollen morphology provides a valuable tool for determining the geographic origins, movements, and seasons of deposition of soil samples, dust, textiles, and products of plant origin, including illegal drugs. By examining pollen in stomach contents, forensic palynologists can help trace the movements and dietary habits of humans and animals.

The study of pollen and related particles such as spores and powdered minerals is known as palynology. As all those who suffer with hay fever know, some flowering plants produce pollen in enormous quantities. Pollen and spores are major components of dust and become incorporated into soil. Two characteristics of pollen make it a valuable tool for the forensic scientist as well as for archaeologists and paleontologists, who first developed techniques of pollen analysis. First, the coating, or exine, of pollen grains resists environmental degradation, and second, pollen’s ornamentation enables many genera and even species to be identified from a single grain. Palynologists have produced catalogs and manuals for identification of pollens and have conducted studies that have linked pollen profiles from soils or air-filtration systems to surrounding vegetation. Large quantities of a pollen type can pinpoint the timing of an event during the flowering season of the parent plant.

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Sampling and Processing

The pollen present on crime victims, at crime scenes, or on suspects or their possessions can provide important evidence. The pollen of anemophilous, or wind-pollinated, plants is most useful to forensic scientists in determining seasonality and general location. When present, the pollen of entomophilous, or insect-pollinated, plants can be used to place a suspect at a specific locality because such pollen requires direct contact for transmission.

Pollen samples need to be rigorously protected from airborne contamination. Because palynological techniques destroy the samples examined, any prior investigations using the same material need to be done in a dust-free environment. As palynology requires specialized equipment and a high degree of expertise, forensic palynological analyses are usually conducted on a consulting basis rather than in crime labs.

Uses of Forensic Palynology

Most applications of forensic palynology involve tracing the origins and movements of people and objects associated with crimes. Pollen in the hair or clothing of a homicide victim, for example, can help pinpoint where the crime occurred. The same spectrum of pollen types found in the clothing of a suspect provides good circumstantial evidence of a connection between the two persons. Rapes, robberies, and other assaults committed around vegetation leave matching pollen traces on the victims and perpetrators. Pollen trapped in a car’s air filter provides a history of where the car has been driven, which can be useful in corroborating or refuting the car’s owner’s presence near a crime scene.

Palynology can be very useful in tracing contraband, including illicit drugs, stolen goods, and illegal imports. Items pick up pollen at the point of origin and at any point where they are stored or repacked. In one case, by analyzing the pollen in several batches of cocaine seized in New York City, investigators determined the batches all came from the same shipment, which had been repackaged in northern New England and further processed in New York City. Pollen lodged in a shipment of oriental rugs indicated that the rugs originated in Iran—not in Egypt, as claimed by the importer.

Marijuana is a prolific pollen producer. Small amounts of Cannabis pollen are not diagnostic, but large amounts in an aerial sample can help law-enforcement authorities locate growing operations. Buildings that have been used for marijuana cultivation, even if thoroughly cleaned, usually harbor quantities of pollen. In addition, investigators can determine the countries and regions where marijuana shipments originated by examining the shipments for the pollen of other plant species.

Although palynology can be a powerful tool in forensic investigations, it is not a tool that is used routinely. One reason for this is that many law-enforcement authorities are unaware of the potential uses of palynology. In addition, highly skilled analysts are needed to perform palynological testing, and the findings of their work are often not conclusive. These drawbacks, coupled with the growing availability of DNA (deoxyribonucleic acid) testing for evidence samples, combine to make palynology a tool of last resort in forensic science. As of the 2020s, the science was only used in a few nations and remained underused in the United States.

Bibliography

Bryant, Vaughan M., John G. Jones, and Dallas C. Mildenhall. “Forensic Palynology in the United States of America.” Palynology 14.1 (1990): 193–208. Print.

Bryant, Vaughan M., John G. Jones, and Dallas C. Mildenhall. “Forensic Studies in Palynology.” Palynology: Principles and Applications. Ed. by Jan Jansonius and D. C. McGregor. Salt Lake City: American Assn. of Stratigraphic Palynologists Foundation, 1996. Print.

Coyle, Heather Miller, ed. Forensic Botany: Principles and Applications to Criminal Casework. Boca Raton: CRC, 2005. Print.

Ezegbogu, Mark O. "Identifying the Scene of a Crime Through Pollen Analysis." Science & Justice, vol. 61, no. 3, May 2021, 205–213, doi: 10.1016/j.scijus.2020.12.002. Accessed 14 Aug. 2024.

Holt, Cynthia. Guide to Information Sources in the Forensic Sciences. Westport: Libraries Unlimited, 2006. Print.

Murray, Raymond C. Evidence from the Earth: Forensic Geology and Criminal Investigation. Missoula.: Mountain, 2004. Print.